Microbiology: MI-502 Techniques in Gene Transfer

Syllabus

Unti-1: Principles of Gene transfer

  1. Bacterial recombination : General principles
  2. Bacterial plasmids- fertility factor
  3. Transfer of plasmid DNA – In vitro plasmid transfer – plasmid replication
  4. Properties of particular bacterial plasmids, f -plasmids, R- plasmids, colicinogenic plasmid -Agrobacterium plasmid Ti –broad host range plasmid
  5. Transposable genetic elements
  6. Insertion sequences- detection of transposition in bacteria – types of bacterial transposons,Mechanism of Replication and non Replicative transposition

Unit-2: Transformation

  1. Discovery of transformation
  2. Biology of transformation
  3. Molecular mechanisms of transformation
  4. Mapping by transformation
  5. Other uses by transformation

Unit-3: Transduction

  1. Generalized transduction
  2. Co transduction & linkage
  3. Mapping by co-transduction
  4. Specialized transduction
  5. Formation of specialized transducing particles from lam bda lysogen
  6. Specialized transduction o f a non lysogen
  7. Specialized transduction of a lysogen
  8. High frequency transuding lysates
  9. Specialized transuding phage as a cloning vehicle

Unit-4: Conjugation

  1. Insertion of F- into the E. coli chromosome HFr trsnsfer
  2. Interrupted matting & time of entry mapp ing
  3. HFr mapping and HFr collection
  4. Mapping “ unselected” Recessive markers
  5. Chromosomes transfer by F+ cultures
  6. Isolation of HFr Strains & F’ plasmids
  7. Chromosomes transfer mediated by F’ plasmids
  8. Rec A- protein & its function