Tests for drinking water sample

several Tests for drinking water sample are explained, learn it from home and answer me.... 1. why Eijkman Got Nobel price? 2. What is application and principle of Elevated temperature test? 3. Why water sample is inoculated in different tubes instead of large vessel, in MPN test? 4. What is procedure of SPC test? 5. Mention limitations of Elevated temperature test, MPN and SPC.
Link to Video: 
Semester: 
3
Unit: 
3
Subject: 
MICROBIOLOGY
Paper: 
2